Lysates are then transferred to the assay plate for the detection of Phospho-VASP (Ser239) by HTRF. FOR HTRF CERTIFIED READER. Remove the plate seal and read on an HTRF compatible reader. SERIAL DILUTIONS . • Low volume (20µl) • Compatible with all HTRF readers • For cell-based or biochemical assays Trying is believing. All requiredThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER For more. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Concentrate on running your assays instead of running your instrument: the optic modules contain all the filters, dichroics, and more needed to run your assay. FOR HTRF CERTIFIED READER For. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. BMG LABTECH’s UV-laser not only allows for faster read times in europium TRF assays, but it also greatly enhances performance in terbium-based TRF assays. Remove the plate sealer and read on an HTRF® compatible reader . Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. overnight at 4 C [Prostaglandin E2] pg/ml. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. White 384-well low volume plate Make sure to use the set-up for Eu 3+ Cryptate. HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER . Quantify up to 10 analytes per well. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. protocol with suspension cells or with a. After an overnight incubation, the plate is washed 3 times. This protocol, HTS designed, allows miniaturization while. For more information about HTRF. The kit includes everything you need: reagents, buffers and a microplate. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Handle complex biological matrices easily. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. FOR HTRF CERTIFIED READER. 25 µL cells + 5 µL compounds + 10 µL supplemented lysis buffer (4X)* + 4 µL HTRF ® pre-mixed antibodies. This protocol, HTS designed, allows miniaturization while. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Read & analyse. The mean and standard deviation. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Detection plates from providers not listed on this. Synergy NEO2 readers must be appropriately configured for HTRF® readout by setting up the measurement conditions in the Gen5™ Reader Control and Data Analysis Software. culture plate. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. Seal the plate and incubate 1 hour at RT : Remove the plate sealer and read on an HTRF® compatible reader . Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. This DLReady-certified microplate reader can also come equipped with Simultaneous Dual Emission for BRET applications and can be further upgraded to a multi-mode reader. 41 mg/ml) was obtained from Thermo Fisher Scientific. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. 96-well culture plateFOR HTRF CERTIFIED READER. 0. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERHTRF® 96-well low volume plate TECHNOLOGY ALL THE BENEFITS OF HTRF WITH THE EASE OF USE OF AN ELISA PLATE!. Plate 25 μL of cells in. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. The LVis Plate is transparent in the UV-range of light and is compatible with all BMG LABTECH microplate readers. The optional HTRF-certified filter-based module may be. Plate 25 μL of cells in. 15 / Each of 1; Qty. HTRF is a proprietary time-resolved fluorescence technology that overcomesThe company offers different HTRF-certified, multidetection microplate readers such as the FLUOstar Omega, POLARstar Omega, CLARIOstar and PHERAstar. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. ** The Detection Buffer is used to prepare working solutions of acceptor and donor reagents. To perform the assay, use white plate only. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYDetection. Lysates are then transferred to the assay plate for the detection of Total Cofilin by HTRF® reagents. • HTRF®-Certified Reader **. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 1. For more. These are the different kinetic modes on BMG LABTECH microplate readers. FOR HTRF CERTIFIED READER. This protocol gives the cells viability and confluence to be monitored. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Figure 3. 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. At that stage, the analyte is sandwiched between the 2 antibodies. As shown in Fig. Whatever the plate type, assay volume should match well volume as closely as possible so that focalization of the reader excitation beam gives maximum energy delivery (Fig. FOR HTRF CERTIFIED READER . TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. HTRF certified . Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the market with all major detection technologies including Alpha and certification for HTRF® technology plus software designed for. (665 nm and 620 nm) on a compatible HTRF ® reader. 9. The optimized well design and high quality plastics provides low background and crosstalk. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. FOR HTRF CERTIFIED READER. For more. It accepts all types of microtitration plate. 1) . No washing steps are required. 27 / Pack of 100; Qty. The PHERAstar ® FSX is our most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. + 4 µL HTRF pre-mixed antibodies . Please note that this kit use a Terbium donor crypate, make sure to set up your reader for Terbium cryptate. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-3 (Ser473) by HTRF. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. It measures 96-well plates in less than 20 seconds and 1536-well formats in under one minute. FOR HTRF CERTIFIED READER For more. Make sure the setup for Eu Cryptate is used. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. Read & analyse. Lysates are then transferred to the assay plate for the detection of phospho- CHK2 (Thr 68) by HTRF. 4 mg/ml. + 4 µL HTRF pre-mixed antibodies . 6% for 1,408 wells and a Z’ value of 0. FOR HTRF CERTIFIED READER culture plate to a 384-well small volume white plate. Fluorescence polarization (FP) is a fluorescence-based detection method that is widely used to monitor molecular interactions in solution. For more information. This protocol gives the cells viability and confluence to be monitored. 96-well culture plate+ 4 µL HTRF pre-mixed antibodies. • HTRF®-Certified Reader. Revvity. The VICTOR Nivo. Unlike standard ELISA plate readers, MSD. Both readers demonstrated their ability to detect IP1 concentrations according to Cisbio’s acceptance criteria. Incubate overnight at RT. com HTRF Microplate Readers. Read other about this measurement approach here. SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. The HTRF cellular assays were found to be at least 16-fold more sensitive than the Western Blot. HTRF 96 TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Add ONE-PLATE ASSAY PROTOCOL: 3h * * Depending on cell lines used, volume of lysis should be optimized. Thanks to the unique Optic Module system, Simultaneous Dual Emission, UV/vis spectrometer, AAS System, and dedicated AlphaScreen ® and. If not, our scientific team will help you choose the instrument that best suits your needs and budget. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). For a list of HTRF-compatible readers and set -up recommendations, please visit. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. For more information about set-up and compatible HTRF ® readers,• HTRF®-Certified Reader. 5 nm filter (PerkinElmer 2100-5110) were used. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations. The tried, tested, and proven EnVision plate reader has an array of exciting features that can enhance your applications. How HTRF can simplify your life The PHERAstar FSX is the best microplate reader for all HTRF assays: Sensitive, fast and reliable measurements with best Z´ and DeltaF% HTRF-certified with white and black plates; Dedicated excitation laser and detectors (PMTs) for best sensitivity; Simultaneous detection of donor and acceptor gives speed and precision See full list on bmglabtech. </p> <p>And, the Enhanced Security software option includes tools to facilitate 21 CFR Part 11 compliance for integration into regulated environments (GxP). FOR HTRF CERTIFIED READERThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. FOR ORE INFORMATION Europe and other ountries 33(0)466 96 0 U. 96-well culture plateONE-PLATE ASSAY PROTOCOL: Small volume detection plate. This protocol gives the cells viability and confluence to be monitored. Add. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. Live Viewer for cell-based assays. PURCHASE SEPARATELY . Phospho-Rb (Ser780) two-plate assay protocol. High-end TR-FRET/HTRF® performance: certified ®to measure HTRF assays in both black or white plates, the reader guarantees no compromises in any HTRF assay. No washing steps are required. If not, our scientific team will help you choose the instrument that best suits your needs and budget. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. Both Microplate Optimization and Read Height Adjustment were. These plates can be read on all HTRF certified readers, thus making their implementation in. Highly compatible plates for HTRF. Plate 25 μL of cells in. Customer Transformation Hub. Lysates are then transferred to the assay plate for the detection of total-p53 by HTRF. HTRF Setup. EnSight ® offers both fast well-imaging and multimode detection capabilities for target-based and phenotypic screening, all in a single benchtop instrument. (2) Forskolin activates the adenylyl cyclase enzyme and increases the intracellular level of cAMP. Eu cryptate emission level). GENERAL LAB WORK USING HTRF KIT 1 Plate 3 mL of cells in 6-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. It features a modular design that allows users to add or change detection cartridges according to their needs. **For more information about HTRF® compatible readers and for set-up recommendations, please visit our website at:ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Read & analyse. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. 96-well or 384-well small volume (SV) detection microplates - For more information about microplate recommendations, please. (HTRF) • Certified for detection of Cisbio’s HTRF assays • GPCR assays (cAMP cell based, membrane based). Plate 25 μL of cells in. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. An. These plates can be read on all HTRF certified readers, thus making their implementation in. Microplate Readers and Reader Accessories. Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for excellence. Neocarzinostatin dose-response on Jurkat cells. Manufacturer. FOR HTRF CERTIFIED READER. For more information about set-up and compatible HTRF® readers,The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. FLUOstar Omega Filter-based multi-mode microplate reader . COMPONENTS ; STORAGE . This protocol enables the cells' viability and confluence to be monitored. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. At Cisbio we provide microplates that help you discover better. The Spark multimode reader from Tecan performs accurate and sensitive measurements of fluorescent cell-based assays. Because of its homogeneous and fast nature, the assay is a preferred method to determine the protein concentration of samples. Incubate 4h at RT. PURCHASE SEPARATELY . FOR HTRF CERTIFIED. STANDARD . Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. It can also be further streamlined toLysates are then transferred ®to the assay plate for the detection of Total TYK2 by HTRF reagents. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. These plates can be read on all HTRF certified readers, thus making their implementation in. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. FOR HTRF CERTIFIED READER. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. But sometimes these parameters are not sufficient enough. Part Number# Product Description Number of Plates# 66PL96001 HTRF 96 well low volume plate 1 plate 66PL96005 HTRF 96 well low volume plate 5 platesThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. The assay is then ready to be read on an HTRF compatible or certified microplate reader. Incubate 4h at RT. February 23, 2010, WINOOSKI VT, USA - The Synergy TM H4 Hybrid Microplate Reader from BioTek Instruments is now HTRF® certified. Synergy H1 offers researchers a choice of monochromators or filters or both detection technologies in one compact unit. For more information about HTRF. Interaction with the industry and research communities is essential for innovating! For more than 30 years, Cisbio has been serving the needs of the clinical and drug discovery communities, delivering a world-class portfolio of assays designed to help find answers faster. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). certified for use with HTRF. Partnerships for delivering turn-key solutions. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Your lab might even have one already. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-2 (Ser473) by HTRF. NC1286225. 96-well culture plateFOR HTRF CERTIFIED READER For more information about HTRF. → Incubate 2h (if the detection is not sensitive enough, it is better to incubate 20h). Dual monochromators for assay flexibility With. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. EM1 616/10 nm EM2 665/10 nm Certified for use with Cisbio’s HTRF Europium Cryptate reagents. Sensitive, accurate and reliable detection is critical across cell, protein and genomic analysis applications in drug discovery and development. Customer Transformation Hub. FOR HTRF CERTIFIED READER For. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. Read & analyse. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. HTRF Signal + 16 µL Standard or Sample 4 µL of pre-mixed TNFα Antibodies ADD READ ANALYSE Incubate 2 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. HTRF® 96-well low volume plate Ref# 66PL96001 * HTRF® 384-well low volume plate Ref 66PL384025 * Non-binding 96-well black plate HTRF®-Certified Reader **. The Negative control is used to check the non-specific signal. Make sure the setup for Tb Cryptate is used. Plate 25 μL of cells in. Filters or monochromators for emission wavelength. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. • HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. The EnVision XCite Plate Reader provides exceptional sensitivity and speed with minimal downtime - backed by our global network of application scientists and engineers. * For HTRF microplate recommendations, please visit . FOR HTRF CERTIFIED. -Certified Reader** *Assay volumes can be adjusted proportionally to run the assay in 96- or 1536-well microplates. Lysis buffer 1X for assay protocol on tissues, two-plate assay protocol on adherent cells, and standard dilution. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). FOR HTRF CERTIFIED READER For. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. FOR HTRF CERTIFIED READER. S. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. The Dual-Luciferase ® Reporter Assay or DLR is widely used to study gene transcription and regulation. FOR HTRF CERTIFIED READER For more information about HTRF. For more ® information about HTRF compatible readers and for set-up recommendations, please. Time-resolved fluorescence was measured switch the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). Assay detection requires a microplate reader with. The lysis buffer 4X is ready-to-use. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Incubate 4h at RT. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesCertified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. For more. use of a wide variety of plates or sample matrix formats. Cover the plate with a plate sealer. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. 68. • User-upgradable microplate reader in under 2 minutes • Array of detection cartridge applications at your fingertips. and TR-FRET, for example to detect PerkinElmer’s DELFIA, HTRF and LANCE assay technologies Time resolved fluorescence certified for use with HTRF. Conclusion. FOR HTRF CERTIFIED READER. Combined with our Kaleido™ workflow software, it. FOR HTRF CERTIFIED READER. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. AlphaLISA SureFire Ultra results are expressed as “Alpha counts”, and HTRF results were expressed as DeltaF, calculated according to CisBio instructions. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. VICTOR® Nivo™ multimode plate reader 2 | For research use only. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. culture plate. The FLUOstar ® Omega is a multi-mode microplate reader with three detection modes. For more information. • Clone your plate data for multiple data reduction optionsExpanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. PLATE READER INTRODUCTION Selecting the right plate reader for your lab can be challenging. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance forThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Other technologies such as imaging. For more information about HTRF. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. VICTOR Nivo Multimode Plate Reader. Detection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Plate 25 μL of cells in. Download the brochure to learn how this compact and robust device can handle various detection modes, plate formats, and software options. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Plate 25 μL of cells in. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non-specific short-lived emissions. No washing steps are required. Make sure the plate reader is turnedon and then open the MikroWin software on the computer. The system provides even higher sensitivity for time-resolved fluorescence (TRF) applications. Lysates are then transferred to the assay plate for the detection of phospho-Tie2 (Tyr992) by HTRF. FOR HTRF CERTIFIED READER. Table 1. Both readers demonstrated their ability to perform the cAMP G s HiRange assay with results well within Cisbio’s required. FOR HTRF CERTIFIED READER. Our microplates and accessories are manufactured under strict process controls guaranteeing consistent product performance for. This protocol, HTS designed, allows miniaturization while. Better sensitivity provides better statistic, less variability among replicates, and a larger assay window. • HTRF®-Certified Reader. FlexStation® 3 Multi-Mode Microplate Reader. Both readers demonstrated their ability to detect IP1 concentrations according to Cisbio’s acceptance criteria. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Our assay development platform relies on PHERAstar readers for absorbance, fluorescence, luminescence, and homogeneous time resolved. For more information. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Incubate at room temperature. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Add ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. FOR HTRF CERTIFIED. Designed to the highest standards to support your drug discovery research, Corning ® and Falcon ® microplates are ideal for drug discovery applications, including assay prep, assay development, and HTS and automation. Step 5 Remove the plate sealer and read on an HTRF® compatible reader The blank control is used to check the Cryptate signal at 620 nm. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. At Cisbio we provide microplates that help you discover better. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. At Cisbio we provide microplates that help you discover better. Read & analyse. Dedicated UV-laser excitation. CulturPlate-96: White Opaque 96-well Microplate, Sterile and Tissue Culture Treated. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for• ®HTRF -Certified Reader(5) (1) IBMX is the most commonly used phosphodiesterase Pan-inhibitor. HTRF Signal + 16 µL 4 µL Standard of pre-mixed IL8 Antibodies or Sample ADD READ ANALYSE Incubate 1 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. 2. Plate 25 μL of cells in. After defining the objectives and their locations in Gen5: 1. The PHERAstar FSX is the best microplate reader for all HTRF assays: Sensitive, fast and reliable measurements with best Z´ and DeltaF% HTRF-certified with white and black. Read & analyse. 55 µL anti-cAMP-Cryptate. View Brochure. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. View Brochure. → Cover the plate with a plate sealer. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. High-end TR-FRET/HTRF® performance: certified ®to measure HTRF assays in both black or white plates, the reader guarantees no compromises in any HTRF assay. Incubate overnight at RT. Microplate readers are only certified if they. 3-in-1 absorbance using a variety of sample formats, including microplates. The plate was covered both incubated for one hour at room temperature. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. To perform the assay, use white plate only. ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes• HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. Data Analysis HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. According to the manufacturers protocol, this protein assay is linear in the range of 0. FOR HTRF CERTIFIED READER. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the. Plate Reader Requirement. Dual monochromators for assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. Lysates are then transferred to the assay plate for the detection of total-Rb by HTRF. The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. Setu CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified. Seal the plate and. HTRF is compatible with a large range of available microplate readers. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. Make sure the setup for Tb Cryptate is used. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Plate 25 μL of cells in. An HTRF assay measures the fluorescence energy transfer between molecules in close proximity. These plates can be read on all HTRF certified readers, thus making their implementation in. • HTRF®-Certified Reader **. The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight at RT * * Depending on cell lines used, volume of lysis should be optimized. It enables you to gain insights you couldn’t achieve with detection measurements alone.